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bio rad cat  (Bio-Rad)


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    Structured Review

    Bio-Rad bio rad cat
    Bio Rad Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 5142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+cat+no+1706516/Goat+Anti-Mouse+IgG+(H+%2B+L)-HRP+Conjugate/bio_rxiv__64898__2026__03__22__713398-114-32-32
    Average 99 stars, based on 5142 article reviews
    bio rad cat - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Combined inhibition of NAD synthesis and C-terminal binding protein cooperatively induces cell death and inhibits growth of high grade serous ovarian carcinoma.
    Article Snippet: Slides were blocked in 5% Goat serum (Thermo Fisher Scientific, Cat No: 31872) for 1h at room temperature and incubated with primary antibodies to CtBP1 (mouse anti-CtBP1, 1:25; BD Biosciences, Cat No: 612042) or CtBP2 (mouse anti-CtBP2, 1:25; BD Biosciences, Cat No: 612044) overnight at 4oC. .. Following three 5 min washes in 1X PBST, the slides were incubated in secondary antibody (Goat Anti-Mouse IgG (H + L)-HRP, 1:200; Bio-Rad, Cat No: 1706516) for 1 h at room temperature and stained using DAB substrate (DAKO Chromogen, Agilent) per manufacturer instructions. .. Nuclei were counter-stained using Mayer’s Hematoxylin (Electron Microscopy Sciences, Cat No: 2617303) for 3 min.

    Article Title: Combined inhibition of NAD synthesis and C-terminal binding protein cooperatively induces cell death and inhibits growth of high grade serous ovarian carcinoma.
    Article Snippet: .. The membranes were then incubated in HRP-conjugated secondary antibodies (goat anti-mouse IgG (H + L)-HRP Conjugate; Bio-Rad, Cat No #1706516; goat anti-rabbit IgG (H + L)-HRP Conjugate, Bio-Rad, Cat No# 1706515; both diluted 1:10000) for 1 h at room temperature. .. After washing 3X for 5 min each in TBST, ECL reagent (SuperSignal West Atto Ultimate Sensitivity Substrate, Cat No# A38554) was added to the membrane and chemiluminescent signal detected using a Bio-Rad Chemidoc MP Imaging system.

    Article Title: Combined inhibition of NAD synthesis and C-terminal binding protein cooperatively induces cell death and inhibits growth of high grade serous ovarian carcinoma
    Article Snippet: .. The membranes were then incubated in HRP-conjugated secondary antibodies (goat anti-mouse IgG (H + L)-HRP Conjugate; Bio-Rad, Cat No: 1706516; goat anti-rabbit IgG (H + L)-HRP Conjugate , Bio-Rad, Cat No: 1706515; both diluted 1:10,000) for 1 h at room temperature. .. After washing 3X for 5 min each in TBST, ECL reagent (SuperSignal West Atto Ultimate Sensitivity Substrate, Cat No: A38554) was added to the membrane and chemiluminescent signal detected using a Bio-Rad Chemidoc MP Imaging system.

    Article Title: Combined inhibition of NAD synthesis and C-terminal binding protein cooperatively induces cell death and inhibits growth of high grade serous ovarian carcinoma
    Article Snippet: Slides were blocked in 5% Goat serum (Thermo Fisher Scientific, Cat No: 31872) for 1 h at room temperature and incubated with primary antibodies to CtBP1 (mouse anti-CtBP1, 1:25; BD Biosciences, Cat No: 612042) or CtBP2 (mouse anti-CtBP2, 1:25; BD Biosciences, Cat No: 612044) overnight at 4 °C. .. Following three 5 min washes in 1X PBST, the slides were incubated in secondary antibody (Goat Anti-Mouse IgG (H + L)-HRP, 1:200; Bio-Rad, Cat No: 1706516) for 1 h at room temperature and stained using DAB substrate (DAKO Chromogen, Agilent) per manufacturer instructions. .. Nuclei were counter-stained using Mayer’s Hematoxylin (Electron Microscopy Sciences, Cat No: 2617303) for 3 min.

    Staining:

    Article Title: Combined inhibition of NAD synthesis and C-terminal binding protein cooperatively induces cell death and inhibits growth of high grade serous ovarian carcinoma.
    Article Snippet: Slides were blocked in 5% Goat serum (Thermo Fisher Scientific, Cat No: 31872) for 1h at room temperature and incubated with primary antibodies to CtBP1 (mouse anti-CtBP1, 1:25; BD Biosciences, Cat No: 612042) or CtBP2 (mouse anti-CtBP2, 1:25; BD Biosciences, Cat No: 612044) overnight at 4oC. .. Following three 5 min washes in 1X PBST, the slides were incubated in secondary antibody (Goat Anti-Mouse IgG (H + L)-HRP, 1:200; Bio-Rad, Cat No: 1706516) for 1 h at room temperature and stained using DAB substrate (DAKO Chromogen, Agilent) per manufacturer instructions. .. Nuclei were counter-stained using Mayer’s Hematoxylin (Electron Microscopy Sciences, Cat No: 2617303) for 3 min.

    Article Title: Combined inhibition of NAD synthesis and C-terminal binding protein cooperatively induces cell death and inhibits growth of high grade serous ovarian carcinoma
    Article Snippet: Slides were blocked in 5% Goat serum (Thermo Fisher Scientific, Cat No: 31872) for 1 h at room temperature and incubated with primary antibodies to CtBP1 (mouse anti-CtBP1, 1:25; BD Biosciences, Cat No: 612042) or CtBP2 (mouse anti-CtBP2, 1:25; BD Biosciences, Cat No: 612044) overnight at 4 °C. .. Following three 5 min washes in 1X PBST, the slides were incubated in secondary antibody (Goat Anti-Mouse IgG (H + L)-HRP, 1:200; Bio-Rad, Cat No: 1706516) for 1 h at room temperature and stained using DAB substrate (DAKO Chromogen, Agilent) per manufacturer instructions. .. Nuclei were counter-stained using Mayer’s Hematoxylin (Electron Microscopy Sciences, Cat No: 2617303) for 3 min.



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